A supplier of laboratory instrumentation for pharmaceutical, biotech, and healthcare sector, TTP LabTech has demonstrated the purpose of Acumen eX3, a laser scanning imaging cytometer, to illuminate cellular pathways that are involved in epidermal growth factor receptor (EGFR) shedding.
Acumen eX3 laser scanning imaging cytometer
The imaging technology providing high throughput of the Acumen eX3 enables sensitive and precise monitoring and quantifying of protein expression levels and has been described in the application note, titled “The use of Acumen eX3 for the identification of the roles of Protein Kinase C (PKC) á & ä isoforms in PMA-induced ectodomain shedding”. The Acumen eX3 exhibits features such as multiplexing capabilities and capturing and analyzing of online images enabling it to offer accurate and consistent information from complicated biological systems.
ADAM17, a metalloproteinase and disintegrin, controls HB-EGF shedding. Acumen eX3’s imaging technology was utilized to identify the role of other enzymatic pathways involved in the HB-EGF shedding process. The HT1080 cells were transformed with a customized alkaline phosphatase linked, heparin binding EGFR (AP-HB-EGF) and were analyzed by traditional enzymatic immunofluorescence and assay for shedding of receptor with or without the presence of inhibitors of PKC isoforms and ADAM17. Experimental results reveals that PKC á & ä isoforms have made key contributions.
Interruption of EGFR homeostasis is related to several important cancer targets, particularly colon, lung, and breast tumors. Therefore, such type of research is important to continue the battle against diseases like cancer.