May 26 2015
DELMIC develops and manufactures products which are focused on high performance, user friendly, integrated microscopy solutions. Working together with Phenom World, the companies have developed the Delphi microscope, the all-in-one solution for Correlative Light and scanning Electron Microscopy.
Together, the companies have hosted workshops in Dresden (MPI-CBG) and Sitges (as part of the 2015 ELMI meeting). June sees the workshop program move to Copenhagen.
A section of 100 nm pancreas rat tissue is labelled for insulin-Alexa594 (orange) and stained with Hoechst (blue), as well as Osmium for extra SEM contrast. In the middle a part of an islet of Langerhans is shown. The islets of Langerhans are the regions of the pancreas that contain endocrine, hormone-producing, cells. These beta cells secrete insulin and play a significant role in diabetes. (Sample courtesy of P de Boer and B N G Giepmans, UMCG Groningen, The Netherlands)
The Delphi microscope is the world's first fully integrated system to provide an all-in-one solution for Correlative Light and scanning Electron Microscopy, CLEM. The design and software of the Delphi make correlative microscopy accessible to fluorescence and electron microscopy users alike. Correlative microscopy is getting more attention for its ability to combine structural and functional information.
However, the complexity of the workflow has so far limited the adoption of the technique by the community at large. Delphi contributes to making the widespread application of correlative microscopy possible. The goals of the workshop are to provide an introduction to CLEM and to show how Delphi has overcome some of the experimental challenges of combining techniques.
The Copenhagen program will feature a talk from Paul Verkade of the University of Bristol. He will talk about applications of high pressure freezing of GFP transfected cells. With his strong background in EM, Dr Verkade has set up a new EM unit as part of the Wolfson Bioimaging Facility, a fully integrated light and electron microscopy centre.
Besides heading the EM unit, he also has his own research group which focuses on the development of techniques and tools for the use of Correlative Light Electron Microscopy to study endocytic sorting. Bristol colleague, Dr Kenton Arkill, will then give a complementary presentation on the direction CLEM is developing including probe development, volume imaging and integrated systems.
This will be supplemented by a talk on high pressure freezing from Leica Microsystems who join Delmic and Phenom World as co-sponsors of the event.
For those wishing to attend, please contact via the event's web site, http://www.delphimicroscope.com/events/workshop-copenhagen.
For more details about DELMIC's SECOM & Delphi systems and applications in the life sciences, please contact DELMIC on +31 (0)15 7440158 or visit the web site: https://www.delmic.com/en/