DynaPro NanoStar II Dynamic Light Scattering Detector

The DynaPro^® NanoStar^®  II is a cuvette-based dynamic light scattering (DLS) instrument used for the analysis of the size and size distributions of peptides, proteins, polymers, nanoparticles and emulsions. A separate, optimized static light scattering (SLS) channel offers true molar mass measurements.

The NanoStar II is among the most sensitive, robust and reliable DLS detectors available for protein and nanoparticle analysis.

Features

Performance

• Sensitivity: 0.1 mg/mL of 14.4 kDa lysozyme in PBS
• Molar mass range: 300 Da to 1 MDa
• Particle Concentration Range: 1x10⁵ to 1x10¹⁵ mL^-1 (dependent on size)
• Size Range R_h by DLS: 0.2 to 1000 nm

Temperature Range

• -10°C to +120°C (0°C to 80°C with disposable cuvettes)
• Maximum ramp rate 15°C/min
• Stability: ± 0.01°C

Sample Options - Sample Cells

• Quartz 2 µL or 45 µL cuvettes
• Disposable 4 µL cuvette (cyclic olefin copolymer)
• Generic 4 mL quartz and disposable cuvettes (with adapter)

Sample Options - Minimum Sample Volume

• 2 µL in quartz cuvette
• 4 µL in disposable cuvette

Optics

• Laser Wavelength: 658 nm
• Laser Power Control: Programmable 10% to 100%
• Scattering Angle: 90º
• Optical Fiber: Multi-mode
• Additional Detectors: Laser monitor for active stabilization
• Additional Detectors: Forward monitor measures transmitted light for turbidance and laser/cell diagnostics

Electronics

• Data Acquisistion Time: 1 to 3600 sec
• Correlator: 512 channel, 100 nsec sampling time in a multi-tau layout

Dimensions: 

• 57 cm (L) x 36 cm (W) x 20 cm (H) 

Applications

• Size, aggregation and stability of small molecules, peptides, proteins, vesicles, nanoparticles, quantum dots and low/sub-micron colloids
• Protein quality control for interaction or structural studies
• Protein and polymer weight-average molecular weight
• Macromolecular interactions: diffusion interaction parameter k_D and second virial coefficient A₂ (B₂₂)
• Thermal onset parameters T_agg, T_onset, T_m
• Solubility of polymers
• Critical micelle concentration and temperature
• Nanoparticle structure by combining MALS and DLS sizing