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Applied Nanoscience Issues Progress Update on NanoFense Protective Face Mask

Applied Nanoscience Inc. (ANI) (PINKSHEETS: APNN), a nanotechnology-based filtration company, today issued a progress update to its investors on a broad array of company efforts, including continued advances toward producing the disposable NanoFense™ Protective Face Mask, designed to offer superior protection against a broad spectrum of harmful virus, bacteria and fungi.

Applied Nanoscience Inc. has completed initial efforts to coat rolls of filter media specified by their Asia Pacific mask partner at an unidentified coating company in the region. This now has accelerated the timeline for supplying the specified hydrophobic media pre-coated with the company's NanoFense formulation, a proprietary antimicrobial nanoparticle filter coating developed by ANI. "This advancement successfully demonstrates a critical internal control which will further protect the company's significant Intellectual Property investment and is a clear indication that ANI is nearing the point of having the ability to supply coated filter media incorporating our technology. This represents the accomplishment of a previously unforeseen security step in the company's role of providing our independently verified technology in a practical and dersirable form," stated Thomas K. Allen, President and CEO - ANI. He is scheduled to leave again for Asia in late January 2010 with the goal of finalizing these efforts.

ANI has also initiated testing efforts at two additional laboratories relating to broadening product claims and new patent application(s). Both testing projects are scheduled for completion by the end of January 2010. In addition, ANI continues to stay focused on U.S. patent efforts tied to its NanoFense formulation as well as the NEFT™ platform.

The revolutionary disposable NanoFense Protective Face Mask will be made available as soon as possible in an effort to help protect people in some of the most heavily populated parts of the world. The company anticipates the reporting of related revenues during the first quarter of 2010. It will be sold in many of the countries where ANI has issued patents protecting their broad NEFT platform. The patent coverage includes three main methods of associating nanoparticles with filter media: (1) coating the filter media with a powder of nanoparticles, (2) impregnating the nanoparticles into the filter media, and (3) having pellets of nanoparticles located adjacent to the filter media.

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