Sep 30 2015
At the 14th Human Proteome Organization World Congress (HUPO), Bruker today announced the new rapifleX MALDI-TOF/TOF mass spectrometer. MALDI-TOF/TOF is established as a powerful tandem mass spectrometry method for in-depth protein analysis.
New rapifleX™ MALDI-TOF/TOF (Photo: Business Wire)
With its redesigned, next-generation TOF/TOF ion optics, the rapifleX system now offers significantly higher speed, mass resolution and mass accuracy.The rapifleX integrates a novel, 10 kHz smartbeam™ 3D scanning laser design for breakthrough mass-spec imaging capabilities with dramatically improved spatial resolution, image contrast and quality, and a new ion source for superior robustness and throughput in pathology research.
Tissue- and cell-type specific proteomics is a major advance for relevant biomarker discovery and identification, and requires MALDI tissue imaging studies which include the identification of detected biomarker candidates for validation and the subsequent development of targeted assays. The rapifleX MALDI-TOF/TOF extends the MALDITissuetyper® imaging capabilities with rapid, spatially resolved MS/MS data. It supports the patented ImageID™ workflow for biomarker discovery in MALDI imaging cohort studies by obtaining matching identifications of tryptic peptides in bottom-up imaging studies.
Dr. Dietrich Hauffe, Executive Vice President for Life Science Mass Spectrometry at Bruker Daltonics, commented: “With the rapifleX MALDI-TOF/TOFintroduction, we can now offer both, greatly enhanced TOF/TOF performance for structural characterization, and breakthrough MS imaging capabilities for cutting-edge proteomics research laboratories, for biopharma customers, and for clinical researchers in pathology. Its unparalleled speed, throughput, structural capabilities, imaging capabilities, as well as MALDI ease of use and robustness, make the new rapifleX MALDI-TOF/TOF a game-changing new research and validation tool.”
About the rapifleX MALDI-TOF/TOF system
In addition to next-generation tissue imaging performance, the rapifleX MALDI-TOF/TOF system is well suited for detailed protein characterization in life science research and biopharmaceutical laboratories. The rapifleX MALDI-TOF/TOF system will improve many protein characterization tasks:
- Peptide mass fingerprints for protein identification
- Fast identification of post-translational modifications (PTM)
- Fast identification of impurities
- Top-down protein sequencing
- T3-Sequencing to identify or confirm suspected modifications or sequence alterations at protein N- or C-termini. T3-Sequencing is patented for protein terminal structure determination.
- Fully automated Disulfide Bond analysis of intact proteins without a priori assumptions using DisulfideDetect™ method and software.
The rapifleX™ MALDI-TOF/TOF system is for research use only.