Bruker is releasing the innovative CaptiveSpray electrospray ion source to suit nano-high performance liquid chromatography (HPLC) systems used in proteomics at ASMS 2011. The use of CaptiveSpray technology ensures significant increase in bottom-up protein identification and delivers optimum, stable sensitivity over long durations.
CaptiveSpray
The Etch-TaperTM technology incorporated in CaptiveSpray enables the spray tip’s internal diameter to remain constant. This lowers tip clogging, and delivers stable operation over extended duration and optimum spray stability over the complete LC gradient.
CaptiveSpray’s main proprietary feature includes an innovative gas-flow focusing technology to deliver high sensitivity gains in comparison with conventional electrospray. The CaptiveSpray source provides nanospray sensitivity, which eliminates the need for making complicated spray tip alterations that will consume time. The novel plug-and-play design of the CaptiveSpray source is compatible with all existing Bruker LC-MS devices.
The CaptiveSpray source is specifically designed to function well with a wide range of LC flow rates as high as 5 uL/min and above or as low as 50 nL/min, which enables analysts to improve overall MS utilization time, to lower column loading time and column re-equilibration. It can operate well at high LC flow rates and deliver high-sensitivity consistently. It improves the loading capacity of samples and widens the dynamic analysis range. Thus, CaptiveSpray source improves the ability of the MS system to identify low-level peptides and to quantify them.